RESUMO
We isolated and analyzed a novel, Gram-stain-positive, aerobic, rod-shaped, non-motile actinobacterium, designated as strain ZFBP1038T, from rock sampled on the north slope of Mount Everest. The growth requirements of this strain were 10-37 °C, pH 4-10, and 0-6% (w/v) NaCl. The sole respiratory quinone was MK-9, and the major fatty acids were anteiso-C15:0 and iso-C17:0. Peptidoglycan containing meso-diaminopimelic acid, ribose, and glucose were the major cell wall sugars, while polar lipids included diphosphatidyl glycerol, phosphatidyl glycerol, an unidentified phospholipid, and an unidentified glycolipid. A phylogenetic analysis based on 16S rRNA gene sequences showed that strain ZFBP1038T has the highest similarity with Spelaeicoccus albus DSM 26341 T (96.02%). ZFBP1038T formed a distinct monophyletic clade within the family Brevibacteriaceae and was distantly related to the genus Spelaeicoccus. The G + C content of strain ZFBP1038T was 63.65 mol% and the genome size was 4.05 Mb. Digital DNA-DNA hybridization, average nucleotide identity, and average amino acid identity values between the genomes of strain ZFBP1038T and representative reference strains were 19.3-25.2, 68.0-71.0, and 52.8-60.1%, respectively. Phylogenetic, phenotypic, and chemotaxonomic characteristics as well as comparative genome analyses suggested that strain ZFBP1038T represents a novel species of a new genus, for which the name Saxibacter gen. nov., sp. nov. was assigned with the type strain Saxibacter everestensis ZFBP1038T (= EE 014 T = GDMCC 1.3024 T = JCM 35335 T).
RESUMO
Gram-stain-negative, aerobic, rod-shaped, non-motile bacterium strain ZFBP2030T was isolated from a rock on the North slope of Mount Everest. This strain contained a unique ubiquinone-10 (Q-10) as a predominant respiratory quinone. Among the tested fatty acids, the strain contained summed feature 8, C14:0 2OH, and C16:0, as major cellular fatty acids. The polar lipid profile contained phosphatidyl glycerol, phosphatidyl ethanolamine, three unidentified phospholipids, two unidentified aminolipids, and six unidentified lipids. The cell-wall peptidoglycan was a meso-diaminopimelic acid, and cell-wall sugars were ribose and galactose. Phylogenetic analyses based on 16S rRNA gene sequence revealed that strain ZFBP2030T was a member of the genus Sphingomonas, exhibiting high sequence similarity to the 16S rRNA gene sequences of Sphingomonas aliaeris DH-S5T (97.9%), Sphingomonas alpina DSM 22537T (97.3%) and Sphingomonas hylomeconis CCTCC AB 2013304T (97.0%). The 16S rRNA gene sequence similarity between ZFBP2030T and other typical strains was less than 97.0%. The average amino acid identity values, average nucleotide identity, and digital DNA-DNA hybridization values between strain ZFBP2030T and its highest sequence similarity strains were 56.9-79.9%, 65.1-82.2%, and 19.3-25.8%, respectively. The whole-genome size of the novel strain ZFBP2030T was 4.1 Mbp, annotated with 3838 protein-coding genes and 54 RNA genes. Moreover, DNA G + C content was 64.7 mol%. Stress-related functions predicted in the subsystem classification of the strain ZFBP2030T genome included osmotic, oxidative, cold/heat shock, detoxification, and periplasmic stress responses. The overall results of this study clearly showed that strain ZFBP2030T is a novel species of the genus Sphingomonas, for which the name Sphingomonas endolithica sp. nov. is proposed. The type of strain is ZFBP2030T (= EE 013T = GDMCC 1.3123T = JCM 35386T).
Assuntos
Sphingomonas , Filogenia , RNA Ribossômico 16S/genética , Sphingomonas/genética , Genômica , Bactérias , Ácidos Graxos , DNARESUMO
To verify the accuracy of MODIS-NDVI data products in deserts and provide guidance for scientific management of desert grasslands in the context of climate change, we examined the responses of fractional vegetation cover (FVC) and normalized difference vegetation index (NDVI) to hydrothermal gradient in arid desert areas using unmanned aerial vehicle (UAV) remote sensing. In Alxa desert region of Inner Mongolia, GreenSeeker handheld spectrometer was used to obtain NDVI (NDVIR) of 100 sampling points. NDVI was extracted by MODIS-NDVI data products (NDVIM), and the accuracy of NDVIM was verified by NDVIR. FVC of each sampling point was obtained through unmanned aerial vehicle remote sensing (FVCU), which was used to examine the FVC that was retrieved by the pixel binary model (FVCM). In addition, combining meteorological data, we examined the responses of FVC and NDVI to hydrothermal gradient based on UAV remote sensing method. The results showed that MODIS-NDVI data products reflected the real NDVI in Alxa area with an accuracy of 84.2%, but NDVIM were 15.7% higher than the actual values. FVCM reflected the vegetation coverage of Alxa region with an accuracy of 83.1%, which were 14.8% lower than the real value. Effects of meteorological factors on NDVI was different, depending on the different acquisition methods. NDVI was affected not only by temperature and precipitation, but also by ground temperature, evaporation and the interaction between evaporation and ground temperature. Because of the different degree of atmospheric influence, NDVIM was more affected by ground temperature, evaporation and precipitation than NDVIR, while NDVIR was more affected by temperature than NDVIM. To examine the changes of vegetation coverage across hydrothermal gradient in desert area, we should consider not only precipitation and temperature, but also the interaction between evaporation, ground temperature and meteorological factors. The interaction between temperature and rainfall, evaporation and ground temperature, and between temperature and evaporation had greater impacts on FVCU.
